Answer:
Correct answer is Saint Bartholomew massacre.
Step-by-step explanation:
Last option is correct as it is only event in which these two countries were not involved. This refers to event in France, when French protestants (Huguenots) were killed during the day of Saint Bartholomew.
All other options are connected with the conflict between these two countries. Drake was the admiral that defeated Spaniards, Mary Stuart was a Catholic ruler of Scotland, while Dutch provinces were part of Spain, and they rebelled against Philip II.