Why is it important to perform a restriction digest after cloning your gene of interest fragments into the TOPO vector?

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asked Aug 9, 2021 231k views

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Alex Kudryashev · Answer 1 · 2021-08-13T15:18:28+0000

Answer:

Restriction enzymes (restriction endonucleases) are proteins that cut DNA at (or close ... To be able to clone a DNA insert into a cloning or expression vector, both have ... you could carry out the two digestions simultaneously (double digestion)

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Why is it important to perform a restriction digest after cloning your gene of interest fragments into the TOPO vector?

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