Question

g why did you digest the plasmid with XhoI? What is your expectation if the plasmid is a closed vector without an insert? What is your expectation if your plasmid has an insert (how many bands, what are their sizes)?

Answer 1

I digest the plasmid with Xholin so as to find out the total size of the plasmid after insertion of the gene of interest and whether or not the insert went into the vector. Ao the plasmid is a reclosed vector without an put we expect to see one band. However, if the plasmid has an insert, we expect to see two bands on the gel