Question

An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated ion exchange chromatography using a cation exchange resin at pH 3.5, with tee buffer at the same pH. Which of the amino acids will be retained on the column? What should be done to elute this amino acid from the column?

Answer 1

The solutions is in the attached file

Step-by-step explanation:

Check the following attached file