9. If the [protein] of the 0.1xE sample was 320 ug/mL, then the concentration in 0.01xE would be 32 mg/ml and the concentration of 1xE would be 3, 200 mg/ma 10. In the example above, what would be the (protein) concentration in the actual 0.1xE protein assay tube that was put in the spectrophotometer? 11. How much of a 1M Tris buffer stock solution should be used to make 250 mL of solution containing 25 mM Tris buffer?