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15 votes
Why do you have to soak the leaf in boiling water after heating it in alcohol?​

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User Jtesch
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2 Answers

3 votes
Starch testing
add the leaf to boiling ethanol in a water bath for a few minutes (the boiling ethanol dissolves the chlorophyll and removes the green colour from the leaf - it turns white so it is easy to see the change in colour)
answered
User JRomio
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7.8k points
5 votes

We boil the leaf in alcohol when we are testing it for starch because to remove the green pigment chlorophyll present in it. For the starch test, we need to observe the colour change from brown to blue when iodine is put on the leaf. But, initially the leaf is green in colour which does not show the required observation. Hence to remove the green pigment present in the leaf we boil the leaf in alcohol when we are testing it for starch.

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User Jgthms
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9.1k points
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