Question

Your supervisor provides you with a 7.2 mg/ml solution of whey antigen. What is the dilution factor if you add 10 uL of 7.2 mg/ml whey antigen to 150 uL of water (dilutent).

Answer 1

The dilution factor is calculated as the total volume divided by the volume of the solution being diluted.

In this case, you are adding 10 uL of the whey antigen solution to 150 uL of water. So, the total volume after dilution is $$10 \, \text{uL} + 150 \, \text{uL} = 160 \, \text{uL}$$.

Therefore, the dilution factor (DF) can be calculated as follows:

$$DF = \frac{\text{Total volume after dilution}}{\text{Volume of solution being diluted}} = \frac{160 \, \text{uL}}{10 \, \text{uL}} = 16$$

So, the dilution factor is 16. I hope this helps! Let me know if you have any other questions.

Step-by-step explanation:

Answer 2

To determine the dilution factor, you need to calculate the ratio of the initial solution's volume (before dilution) to the final solution's volume (after dilution).

Initial volume of whey antigen solution (V1) = 10 uL
Final volume of diluted solution (V2) = 10 uL + 150 uL = 160 uL

Dilution factor (DF) = V1 / V2
DF = 10 uL / 160 uL
DF = 1/16

So, the dilution factor is 1/16, which means you have diluted the original 7.2 mg/ml whey antigen solution by a factor of 1/16 when you added 10 uL to 150 uL of water.