Final answer:
HPTLC is an advanced form of planar chromatography where samples are applied onto a stationary phase and separated using a mobile phase drawn up by capillary action. It offers advantages like simultaneous analysis of multiple samples, low solvent usage, and wide range of detection methods. It is faster, more accurate, and reliable due to smaller particle size and advanced instrumentation.
Step-by-step explanation:
High-Performance Thin-Layer Chromatography (HPTLC) is an established technique in the field of analytical chemistry. It is a type of planar chromatography where the substances are separated on a thin layer of stationary phase, typically a plastic foil or a glass plate covered with silica gel, cellarose or polyamid, and the mobile phase is drawn up to the stationary phase's designated path.
In summary, the main answer to the procedure involves the application of sample spots onto the TLC plate using a micropipette. The plate is then placed in a development chamber containing the mobile phase, which travels up the plate by capillary action, causing separation of the substances. After the developing process, the plate is dried and examined under UV light to observe the separate substances.
There are several advantageous to using HPTLC: it allows for many samples to be analyzed simultaneously, it consumes less solvent than other chromatography techniques, and it allows for a wide range of detection methods. These advantages make HPTLC a highly efficient method of analysis.
In essence, HPTLC is a sophisticated version of TLC with the advantages of being faster, providing higher resolution due to smaller particle size, and being more accurate and reliable because of advanced instrumentation and detection methods.
Learn more about HPTLC