Question

estion 8 What technique is often used today to identify whether mutation(s) exist in particular bacterial genes? site-directed assaying sequencing Southern and/or Northern blotting functional screening Click Submit to complete this assessment.

Answer 1

Site-directed mutagenesis is the technique used today to identify mutation(s) in particular bacterial genes.

Step-by-step explanation:

The technique used today to identify whether mutation(s) exist in particular bacterial genes is site-directed mutagenesis. This technique allows researchers to introduce specific mutations into a gene of interest and observe the resulting effects on the phenotype. By selectively altering nucleotide sequences, scientists can determine the function and role of specific genes in bacterial organisms.

Answer 2

Southern blotting is a prominent technique used today to identify mutations in particular bacterial genes. It involves transferring DNA fragments onto a nylon membrane and probing with labeled DNA sequences to detect specific mutations.

Step-by-step explanation:

Techniques to identify mutations in bacterial genes have advanced significantly. A key method used today is Southern blotting, which is a powerful technique developed by Sir Edwin Southern in 1975. The process involves separation of DNA fragments via agarose gel electrophoresis, their transfer to a nylon membrane, and subsequent detection using a specific single-stranded DNA probe that is labeled with a radioactive or fluorescent beacon. This process effectively detects the presence of certain DNA sequences and can be instrumental in identifying particular mutations in bacterial genes.

Northern blotting is another related technique but is used to examine RNA to assess gene expression. Other methods like site-directed mutagenesis, sequencing, and functional screening also play a role, but for the detection of specific DNA sequences within a genome, Southern blotting remains a highly utilized method.