Final answer:
Seville is the city in Spain that is located on the Guadalquivir river, the largest city of Andalusia, and the only river port of Spain, with the Alcazar Palace.
Step-by-step explanation:
The city in Spain that is located on the Guadalquivir river, the largest city of Andalusia, and the only river port of Spain, with the Alcazar Palace, is Seville.
Seville is a historic city known for its rich cultural heritage, including the Alcazar Palace, which is a UNESCO World Heritage Site.
The Guadalquivir river runs through the city, and Seville has a thriving river port.