Final answer:
The concept of Free-Running of the Pacemaker in Biological Rhythms refers to the pacemaker running freely without any external cues or synchronization. Pacemaker cells in the heart's SA node generate electrical signals that control the heart rate, which can operate independently of external cues.
Step-by-step explanation:
The concept of Free-Running of the Pacemaker in Biological Rhythms refers to option 1) The pacemaker runs freely without any external cues or synchronization. The pacemaker cells, located in the heart's sinoatrial (SA) node, are self-excitable and generate electrical signals that control the heart rate. These signals can operate independently of external cues or synchronization, allowing the pacemaker to run freely and maintain the heart's rhythm.