Question

Describe the steps of generating transgenic mice by pronuclei injection?

Answer 1

The steps of generating transgenic mice by pronuclei injection involve obtaining and preparing donor eggs and sperm, injecting foreign DNA into the male pronucleus of a zygote, implanting the modified zygote into a surrogate female mouse, and allowing the surrogate female to give birth to transgenic mice.

Step-by-step explanation:

Obtain and prepare donor eggs and spermInject a foreign DNA into the male pronucleus of a zygoteImplant the modified zygote into a surrogate female mouseAllow the surrogate female to give birth to transgenic mice

Pronuclei injection involves inserting a foreign DNA into the male pronucleus of a zygote. The modified zygote is then implanted into a surrogate female mouse, who gives birth to transgenic mice.