Question

Controlled rate freezing is conducted with which of the following conditions:

a. The employment of computer assisted freezing
b. The addition of cryoprotectant and freezing at a predetermined rate per minute
c. Use of ethylene glycol in the freezing system
d. Store in the liquid hydrogen

Answer 1

Controlled rate freezing is conducted with the addition of cryoprotectant and freezing at a predetermined rate per minute.

Step-by-step explanation:

The correct answer is b. The addition of cryoprotectant and freezing at a predetermined rate per minute.

Controlled rate freezing involves adding a cryoprotectant (such as glycerol or ethylene glycol) to the biological material to prevent ice crystal formation, which can damage the cells. The freezing process is then conducted at a predetermined rate per minute to ensure slow and controlled cooling. This helps to maintain the integrity of the cells during freezing and thawing.

Answer 2

Controlled rate freezing is conducted with the addition of cryoprotectant and freezing at a predetermined rate per minute.

Step-by-step explanation:

The correct answer is b. The addition of cryoprotectant and freezing at a predetermined rate per minute.

Controlled rate freezing involves adding a cryoprotectant (such as glycerol or ethylene glycol) to the biological material to prevent ice crystal formation, which can damage the cells. The freezing process is then conducted at a predetermined rate per minute to ensure slow and controlled cooling. This helps to maintain the integrity of the cells during freezing and thawing.

Answer 3

Controlled rate freezing is conducted with the addition of cryoprotectant and freezing at a predetermined rate per minute.

Step-by-step explanation:

The correct answer is b. The addition of cryoprotectant and freezing at a predetermined rate per minute.

Controlled rate freezing involves adding a cryoprotectant (such as glycerol or ethylene glycol) to the biological material to prevent ice crystal formation, which can damage the cells. The freezing process is then conducted at a predetermined rate per minute to ensure slow and controlled cooling. This helps to maintain the integrity of the cells during freezing and thawing.