Question

Summarize the appropriate procedure for obtaining a pure culture from the growth of an isolated colony on a selective medium

Answer 1

The appropriate procedure for obtaining a pure culture from the growth of an isolated colony on a selective medium involves checking for isolated colonies, transferring them to the refrigerator, and subculturing a chosen colony onto a TSA plate.

Step-by-step explanation:

To obtain a pure culture from the growth of an isolated colony on a selective medium, the appropriate procedure is as follows:

1. After incubation, check if isolated colonies have developed on the plate. If there are isolated colonies, transfer the plate to the refrigerator.
2. Choose one single colony from the plate and prepare a pure culture.
3. Subculture the chosen colony to a section of a TSA plate to create a pure culture.

Incubate the plate until bacterial growth is abundant. This process ensures that the culture contains only one type of bacteria.