Question

What are potential sources of error in gel electrophoresis?

Answer 1

Potential sources of error in gel electrophoresis include spontaneous errors by DNA polymerase, exposure to gamma radiation, exposure to ultraviolet radiation, and exposure to intercalating agents.

Step-by-step explanation:

Potential sources of error in gel electrophoresis include:

1. Spontaneous errors by DNA polymerase, which can lead to incorrect amplification of DNA fragments.
2. Exposure to gamma radiation, which can damage the DNA fragments and affect their migration through the gel.
3. Exposure to ultraviolet radiation, which can cause crosslinking of the DNA fragments and alter their mobility during electrophoresis.
4. Exposure to intercalating agents, which can bind to the DNA and change its conformation, affecting its migration through the gel.