Final answer:
Veterinary histology utilizes staining, fixation, and sectioning to prepare tissue samples for microscopic examination, with techniques varying between light microscopy, TEM, and SEM.
Step-by-step explanation:
In veterinary histology, different techniques are employed to prepare tissue samples for microscopic examination. These include staining, fixation, and the creation of thin sections for both transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Stains and fluorescent dyes assist in differentiating tissue components. Preparing specimens can involve making wet mounts for immediate observation or fixing samples to preserve cellular details.
Among the staining techniques utilized are Gram staining, acid-fast staining, capsule staining, endospore staining, and flagella staining. Samples for TEM must be sliced into very thin sections, while SEM requires a process known as sputter-coating to make the surface of the specimen conductive.
To accurately categorize tissues under the microscope, such as distinguishing between simple and complex tissues or identifying plant tissues as monocot or dicot, students need to be well-versed in tissue types and their microscopic characteristics.