Question

When you extract the yeast cells using the "smash and grab" technique with chloroform, where does the DNA fractionate to?

A) Aqueous phase
B) Organic phase
C) Interface
D) Solid phase

Answer 1

In the 'smash and grab' technique with chloroform, the DNA fractionates to the aqueous phase and is then precipitated with ethanol.

Step-by-step explanation:

When extracting yeast cells using the 'smash and grab' technique with chloroform, the DNA fractionates to the aqueous phase. This is the phase where the DNA remains after the extraction process. It is then precipitated with ethanol to form viscous strands that can be spooled on a glass rod.