Final answer:
In the spectrophotometric assay used in this experiment, the absorbance increases as the glucose concentration in the sample increases.
Step-by-step explanation:
In the spectrophotometric assay used in this experiment, the absorbance increases as the glucose concentration in the sample increases. The assay uses a color-changing reaction that is directly proportional to the amount of glucose present. As the glucose concentration increases, more color is produced, leading to a higher absorbance reading. This allows for the quantification of glucose concentration in the sample.