Question

Which of the following is not necessary for RNA polymerase to recognize the promoter of a bacterial gene?

1) TATA box
2) -35 region
3) -10 region
4) Shine-Dalgarno sequence

Answer 1

The TATA box, -10 region, and -35 region are necessary for RNA polymerase to recognize the promoter of a bacterial gene, but the Shine-Dalgarno sequence (option 4) is not.

Step-by-step explanation:

The TATA box, -10 region, and -35 region are all necessary for RNA polymerase to recognize the promoter of a bacterial gene. The TATA box is a specific DNA sequence that helps initiate transcription. The -10 region and -35 region are promoter consensus sequences that are recognized and bound by sigma factors. However, the Shine-Dalgarno sequence is not necessary for RNA polymerase to recognize the promoter of a bacterial gene. The Shine-Dalgarno sequence is found in the 5' untranslated region of mRNA and is responsible for ribosome binding in prokaryotes, not promoter recognition.