Final answer:
In Gel Filtration Chromatography, small proteins are eluted later as they enter the pores of the chromatography beads, while larger proteins are excluded and eluted faster.
Step-by-step explanation:
In Gel Filtration Chromatography, also known as size exclusion chromatography, proteins are separated based on their size. The porous beads within the chromatography column allow small proteins to enter their pores, while larger proteins are excluded and travel faster through the column.
Consequently, proteins small enough to enter the pores are eluted later than the larger proteins that are excluded from the pores. This separation technique is based on the principle that smaller molecules take a longer path through the matrix, resulting in delayed elution times compared to larger molecules that are excluded from the pores and therefore eluted faster.