Question

Because DNA differs for individuals, adding a restriction enzyme to a sample can create multiple cuts that are

unique to each sample.
1) Examine the sequences below and indicate where the Smal enzyme cuts the DNA and how many
fragments are created
A T C A T C C C G G G A G A G C T A G C C C G G G A A A T A G G C C C G G GAT CATGA T T
T A G T A G G G C C CTCTC G A T C G G GCCCTТТАТСC GGGCC C T A G T A C T A A
How many fragments are created?
AA C
A T GAA CATC C C G G G A T C A A G G C A G G A A A C C C G G G AT A G T T A A C C
T I G
T A C T T G T A G G G C CCTAGTтссстостттссссс СТАТ С A A T T G G
How many fragments are created?

Answer 1

For the first sequence, the Smal enzyme cuts at the following sites:

ATC|ATC

AGC|CCG

GGA|TAG

CCC|GGG

ATA|GGC

CCCGGG|ATC

ATTTAG|CTG

This creates 7 fragments.

For the second sequence, the Smal enzyme cuts at the following sites:

TGAAC|ATCC

CGGG|ATTAGT

TCCC|TAG

TAACTT|GTAGG

GCC|CTAGT

TTCAG|GCC

TTA|TTGG

This creates 7 fragments.