Mitosis is the process that facilitates the equal partitioning of replicated chromosomes into two identical groups. Before partitioning can occur, the chromosomes must become aligned so that the separation process can occur in an orderly fashion. The alignment of replicated chromosomes and their separation into two groups is a process that can be observed in virtually all eukaryotic cells. Interfase must happen before mitosis can begin. Interfase is when the parent cell stores energy and becomes bigger so it can make the two daughter cells.