Question

A scientist has inserted a gene into a plasmid. Which enzyme should she use to join the sticky ends of the gene and the plasmid?

Answer 1

You would use DNA ligase to join the sticky ends together. 

Answer 2

The enzyme you should use is DNA ligase

Step-by-step explanation:

The first step for cloning is to cut the plasmid and paste the gene of interest inside. This process is dependent on the restriction enzymes and the enzyme DNA ligase, which is responsible for binding the DNA.

The enzyme DNA ligase catalyzes the attachment of the insert and the vector through a plasmid, with the formation of covalent bonds.