Gram-positive microorganisms have a thicker layer of peptidoglycan which traps a greater amount of the gem violet included and upon de-colorizer discharges approximately caught stains.
Gram-positive cell dividers contain a thick peptidoglycan layer with teichoic acids. Gram-negative cell dividers contain a thin peptidoglycan layer that is encompassed by a thick plasma film. Gram-positive microorganisms will recolor purple in view of their thick peptidoglycan cell divider.