Question

The steps of the process of creating recombinant DNA are listed below. Put them in order from first (1) to last (5). Vector and recombinant DNA multiply. Selected gene is isolated. Recombinant DNA is inserted into host. Fragments of DNA are inserted into a vector. Restriction enzymes cut DNA into fragments.

Answer 1

1. Selected gene is isolated.
2. Restriction enzymes cut DNA into fragments.
3. Fragments of DNA are inserted into a vector.
4. Vector and recombinant DNA multiply.
5. Recombinant DNA is inserted into host.

Answer 2

The appropriate sequence of steps involved in recombinant DNA technology are as under :-

1) Selected gene is isolated.

2) Restriction enzymes cut DNA into fragments.

3) Fragments of DNA are inserted into a vector.

4) Vector and recombinant DNA multiply.

5) Recombinant DNA is inserted into host.

In the very first step, the desired gene/genetic material for cloning is isolated from the cell. To do this the cell is lysed. Cells can be lysed by sonication or using chemicals like detergents.

In the second step, isolated DNA/gene is cut with the help pf biological scalpels known as restriction enzymes. The vector (usually it is a plasmid) is also cut down using restriction enzymes.

In the third step, the fragment of the restriction digested DNA (gene) and the vector are ligated together.

In the fourth step, this vector in which desired gene has been incorporated is cloned to generate various copies.

In the last step, this recombinant DNA is inserted into a host cell where is expressed i.e. in host cell this desired gene produces its product (protein).