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Adipose connective tissue cells are specialized for fat storage and do not form ground substance or fibers. On prepared slides, adipose tissue appears somewhat like a fish net with white spaces connected together in a network. The cytoplasm and nucleus have been pushed to one side by a single, large, fat-filled vacuole that occupies the center of the cell. In this cross section from the trachea, the adipose cells are located in the outer most layer (adventitia) of the organ.