Question

You have made a smear of a bacterial culture and have performed the Gram stain on it. Looking at the organism under the microscope, you notice that the cells do not seem to be the dark blue-purple of a gram-positive reaction, but instead are light blue. Your staining procedure was performed correctly. What is your best explanation as to why the bacteria have stained this way?

Answer 1

The way the dark blue is smeared is thin so it goes light when spread out