Question

Robert Koch helped establish the germ theory of disease by discovering that anthrax was caused by a bacteria. After he isolated and purified the same bacteria from several diseased animals, what would be the next step in order to show that this bacteria caused anthrax?

Answer 1

Inoculate them in healthy animals.

Step-by-step explanation:

Koch could hypothesize that the bacteria he isolated from sick animals was the cause of Anthrax; however, he could only confirm this by inoculating the isolated bacteria into healthy animals expecting them to get sick as well. After they got sick, he had to again isolate the same bacteria from them.