Question

Which of the following is required for repairing the phosphodiester backbone of DNA during molecular cloning?

cDNA
reverse transcriptase
restriction enzymes
DNA ligase

Answer 1

DNA ligase.

Step-by-step explanation:

DNA ligase is the enzyme required to reseal the DNA molecule. This enzyme causes the formation of phosphodiester bonds between the DNA molecule.

DNA ligase is used in different molecular technologies like DNA cloning and recombinant DNA technology. This enzyme repairs the phosphodiester bond during molecular cloning of DNA.

Thus, the correct answer is option (4).