Answer:
Lysis, precipitation and purification
Step-by-step explanation:
DNA isolation is a molecular biology technique based on the extraction of DNA and subsequent purification to remove inhibitors that may hamper the process of amplification. DNA isolation consists of three steps:
1- Lysis: it consists of the mechanical disruption of the cell, where the nucleus is broken to release DNA. During this process, it is required to use enzymes and detergents such as Proteinase K
2- Precipitation: during this process, the DNA is precipitated by using alcohol and salt.
3- Purification: this process removes the impurities from DNA. Extraction is carried out by washing DNA with ethanol and water.