Question

A fusion protein can be sythesized to carry a specific tag, which is useful in identifying the proteins in various experiments. Fusion proteins with the FLAG epitope bind to specific anti-FLAG antibodies and carry this extra sequence:

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
At a pH of 7.0 the amino acids that would be charged in FLAG are:______.
A. Tyr.
B. Lys.
C. Asp.
D. Glu.

Answer 1

B and C

Step-by-step explanation:

At a pH level of 7, aspartic acid has a net charge that is of -1, that is it is negatively charged.

While lysine has a net charge of +1 at this same pH level of 7

Tyrosine on the other had has zero net charge at this pH. And glutamine itself is absent.

Therefore the amino acids that are going to be charged are :-

b. Lys

c. Asp