Question

You plate colonies of E. coli with the lac operon genotype of I-P+O+Z+CAP+ on 1) a medium containing only glucose, and 2) a medium containing only lactose. You isolate mRNA from bacteria from each medium and do a Northern blot with a probe for β-galactosidase. Draw the results.

Answer 1

A complete collection of single-use and high-throughput electrocompetent and chemically competent E. coli

Choosing the ideal competent cells for your cloning applications and workflows is a critical component of success. Aspects to consider when selecting your competent E. coli strain include:

Transfection method – chemical versus electroporation

Transformation efficiency – bacterial strain and genotype

Downstream application – protein expression, library production, general cloning