Question

6. What are the two ingredients found in the lysis solution? ​

Answer 1

The two ingredients found in the lysis solution are sodium hydroxide (NaOH) and potassium hydroxide (KOH). These alkaline substances are used to break the cell membrane and release the plasmid DNA.

Step-by-step explanation:

The two ingredients found in the lysis solution are sodium hydroxide (NaOH) and potassium hydroxide (KOH). These are alkaline substances that are used to break the cell membrane and release the plasmid DNA during plasmid preps. The lysis solution can be made by mixing these ingredients with other components, such as buffers and enzymes, depending on the specific protocol.

Answer 2

Detergent and Proteinase K:

Explanation:

The detergent disrupts the cell membrane and nuclear envelope, causing the cells to burst open and release their DNA. The proteinase K cuts apart the histones to free the DNA.

Hope this helps!