Question

4.) The DNA sequence above is found on a chromosome in a eukaryotic cell. The cell is allowed to undergo DNA replication and mitosis in a solution containing radio-label free nucleotides. These nucleotides are similar to a normal free nucleotides found in the cell, but contain a radioactive atom.

What is the sequence of the new strand formed by DNA polymerase?

Answer 1

since DNA polymerase requires free 3'OH group for initiation of synthesis, it can only synthesize and One Direction by extending the 3' end of the preexisting nucleotide chain hence DNA polymerase moves along the template strand in a '3-5' direction, and the daughter strand is formed in a '5-3' direction.