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How might you distinguish between these possibilities? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. ResetHelp If repressor inhibits RNA polymerase binding to the promoter, then one way to demonstrate this would be to incubate a plasmid containing the lac operon with lac If repressor inhibits R N A polymerase binding to the promoter, then one way to demonstrate this would be to incubate a plasmid containing the lac operon with lac blank. Then add R N A polymerase and subject the mixture to sucrose gradient centrifugation and analyze each fraction for R N A polymerase and repressor, using antibodies to the two proteins. A simpler method would be to analyze the mixture by gel electrophoresis and probe the gel ( blank ) with antibodies to R N A polymerase and repressor ( blank ). Another experiment could involve footprinting; carry out blank assays, asking whether repressor inhibits R N A polymerase binding or vice versa.. Then add RNA polymerase and subject the mixture to sucrose gradient centrifugation and analyze each fraction for RNA polymerase and repressor, using antibodies to the two proteins. A simpler method would be to analyze the mixture by gel electrophoresis and probe the gel (

asked
User Amisha
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7.7k points

1 Answer

4 votes

Answer:

all the given blanc spaces will be filled accordingly as below

  1. Repressor
  2. western blot
  3. in separate gels
  4. competitive binding

Step-by-step explanation:

answered
User Ilya Zinkovich
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8.6k points
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