Question

Changing the pH of the binding reaction mixtures can have a dramatic effect on ligand-protein binding. Altering the pH of the reactions between warfarin (which binds to site IIA of HSA via hydrophobic interaction) or ibuprofen (which binds to site IIIa of HSA via an ionic interaction) and HSA is likely to:A. Affect both reactions equallyB. Affect warfarin-HSA binding but not ibuprofen-HSA bindingC. Affect ibuprofen-HSA binding but not warfarin-HSA bindingD. Not affect either interaction

Answer 1

B

Step-by-step explanation:

Answer 2

B

Step-by-step explanation:

Changing the pH of the binding reaction mixtures can have a dramatic effect on ligand-protein binding. Altering the pH of the reactions between warfarin (which binds to site IIA of HSA via hydrophobic interaction) or ibuprofen (which binds to site IIIa of HSA via an ionic interaction) and HSA is likely to Affect warfarin-HSA binding but not ibuprofen-HSA binding.