Question

Below is the sequence of the coding strand of DNA that flanks the coding region of the GFP gene. Diagram of the DNA coding strand with this sequence: 5'-ATCGG--GFP gene--TTAGAC-3' Select the sequence of the forward primer that you would use to amplify the GFP gene using PCR. Remember that the forward primer hybridizes to the template strand of DNA. Select the sequence of the forward primer that you would use to amplify the GFP gene using PCR. Remember that the forward primer hybridizes to the template strand of DNA. 5' GTCTAA 3' 5' TAGCCC 3' 5' ATCGGG 3' 5' TTAGAC 3'

Answer 1

The answer is 5’ ATCGGG 3’

Step-by-step explanation:

The term coding strand refers to the DNA strand whose sequences correspond to the RNA transcript produced. This s the strand which is complementary to the gene or DNA strand from which mRNA is synthesized. Information is given that the DNA sequence provided is coding strand and hence, the gene has complementary sequences to that.

So then, the primers used to amplify the gene will be of same orientation of the coding strand given.

The correct answer is 5’ ATCGGG 3’