Question

If a restriction enzyme has as its recognition sequence "ATCCTA," how many restriction sites would appear in the lambda genome, which is approximately 54,000 bp?

Answer 1

The number of restriction sites that would appear in lambda genome which is of 54,000 bp is 13.

Step-by-step explanation:

The number of times a restriction enzyme can cut in a DNA sequence is one time for every 4ⁿ, where n is the total number of bases in that particular recognition sequence of the restriction enzyme. In this case the recognition sequence is ATCCTA which contains 6 bases.

So 4×4×4×4×4×4= 4056

Size of the genome= 54,000 bp

Number of restriction site= 54000÷4056 = 13.1 or 13