Question

Explain how the results from testing Barley High Lysine (BHL) are related to the primary, secondary and tertiary structure of protein?

Answer 1

Enhanced derivatives of barley chymotrypsin inhibitor-2 are known as Barley high lysine (BHL) proteins. BHL has tryptophan, threonine, and isoleucine in a folded structure similar as the tertiary structure of protein.

Derivatives of BHL are digestible in gastric juice and other fluid. Denaturation of these proteins shows proteins in gastric fluid minus pepsin which is looks as the secondary structure of protein. It has four soluble protein groups –

a) Albumins

b) globulin fraction in embryo and scutellary proteins

c) Prolamins

d) Glutelin