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When developing a complementation assay to identify proteins involved in dna synthesis, what is used to make the in vitro assay of dna synthesis?

When developing a complementation assay to identify proteins involved in dna synthesis, what is used to make the in vitro assay of dna synthesis?
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when developing a complementation assay to identify proteins involved in dna synthesis, what is used to make the in vitro assay of dna synthesis?

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User Joeblog
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Heat treated extract prepared from the temperature sensitive conditional mutant.

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User Arshpreet
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