Question

assume that you are trying to insert a gene into a plasmid. someone gives you a preparation of genomic dna that has been cut with restriction enzyme x. the gene you wish to insert has sites on both ends for cutting by restriction enzyme y. you have a plasmid with a single site for y, but not for x. your strategy should be to

Answer 1

Your strategy should be to cut the DNA again with a restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.