Why it is necessary to use the same restriction enzyme to cut the desired gene from the source and to cut the plasmid

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asked Nov 20, 2023 127k views

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Josh Newman · Answer 1 · 2023-11-26T02:22:58+0000

Answer:

The restriction enzymes cuts at fixed sequences, therefore same restriction enzyme must be used since it forms fragments with complementary sticky ends, therefore facilitating the formation of bonds between them.

Step-by-step explanation:

Why it is necessary to use the same restriction enzyme to cut the desired gene from the source and to cut the plasmid

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