Question

What is the Effect of pH on Catalase Activity

1. Add 2 ml hydrogen peroxide to 4 clean test tubes, then add:
Tube 1 – add 3 drops of acetic acid (vinegar) pH =_______
Tube 2 – add 3 drops of sodium bicarbonate (base) pH =______
Tube 3 – add 3 drops of water (neutral) pH =_____
Tube 4 -- add 3 drops of 1M NaOH pH = _____

Answer 1

Changes in pH do not only affect the shape of the catalase enzyme but also change the shape or charge properties of the hydrogen peroxide substrate so that the hydrogen peroxide will not bind to the active site and thus cannot undergo catalysis.

Step-by-step explanation: