What role does dna ligase perform in a dna cloning experiment? how does the action of dna ligase differ from the function of restriction enzymes?

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asked Jun 12, 2023 210k views

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Alex Chadyuk · Answer 1 · 2023-06-18T12:36:47+0000

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In a DNA cloning experiment, DNA ligase is used to generate the covalent bonds of the phosphodiester backbone to yield an intact double-stranded DNA molecule. Restriction enzymes, on the other hand, break such bonds.

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What role does dna ligase perform in a dna cloning experiment? how does the action of dna ligase differ from the function of restriction enzymes?

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