Question

You physically puncture the thylakoid membranes. This puncturing does not damage any of the molecules bound to the thylakoid membrane (i.e. they still function normally), but now there are large holes connecting the thylakoid space and stroma. How will this affect the light-harvesting reactions?

Answer 1

If the thylakoid membranes have large holes connecting the thylakoid space and stroma, it can disrupt the normal flow of ions and create a leak. This can affect the light-harvesting reactions by reducing the efficiency of ATP and NADPH synthesis.