Question

Determine these three dilutions:

Dilution 1:4x102

Dilution 2: 0.002%

Dilution 3: 150uM

with the following equation y=622x.

from a 1/50 dilution of methelyne blue at 0.26%



given values:

absorbance for dilution 1: 0.700

absorbance for dilution 2: 1.711

absorbance for dilution 3: 3.210



Theoretical amount for 1

.26% (m/v) methylene blue : 2.5µL

Water: 997.5 µL



Theoretical amount for 2

.26% (m/v) methylene blue : 7.7µL

Water: 992.3 µL



Theoretical amount for 3

.26% (m/v) methylene blue : 18.5µL

Water: 981.5 µL

Answer 1

determine these three dilutions Dilution 1 4x102 Dilution 2 0.002 Dilution 3 150uM with the following equation y 622x from a 1 50 dilution of methelyne blue at 0.26 given values absorbance for dilution 1 0.700 absorbance for dilution 2 1.711 absorbance for dilution 3 3.210 Theoretical amount for 1 26 m v methylene blue 2.5µL Water 997.5 µL Theoretical amount for 2 26 m v methylene blue 7.7µL Water 992.3 µL Theoretical amount for 3