1. Immunohistochemical analysis is a technique used in histology to visualize specific proteins or antigens within tissues using antibodies. It involves using labeled antibodies that bind to target proteins, allowing researchers to identify and locate specific molecules within cells or tissues.
2. Microtubules are composed of tubulin protein subunits arranged in a cylindrical structure. The building blocks of microtubules are tubulin dimers, which consist of α-tubulin and β-tubulin subunits. A protofilament is a linear chain of tubulin dimers that makes up the wall of a microtubule. Multiple protofilaments align side by side to form the tubular structure of microtubules.
3. Hematoxylin and eosin (H&E) are common stains used in histology to color different structures within tissues. Hematoxylin stains nuclei and other acidic structures blue-purple, while eosin stains cytoplasm and other basic structures pink. This staining technique helps reveal cellular details and differentiation in tissue samples.