Question

You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data

and observational data you acquired, re-calculate the number of CFUS in the original undiluted sample.
Number of CFUS =
CFUS

Answer 1

To calculate the number of Colony Forming Units (CFUs) in the original undiluted sample, we need to consider the dilution factor caused by the incorrect inoculation.

First, let's determine the dilution factor. Since the plate was inoculated with only 0.1mL instead of 1mL, the dilution factor is 1/0.1 = 10.

Next, we need to calculate the CFUs in the diluted sample on the plate. Let's assume you counted 50 CFUs on the plate.

To find the CFUs in the original undiluted sample, we multiply the number of CFUs on the plate by the dilution factor:

Number of CFUs = CFUs on the plate x Dilution factor

= 50 CFUs x 10

= 500 CFUs

Therefore, the number of CFUs in the original undiluted sample is 500.

Step-by-step explanation:

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