This is the order of the genes on chromosome II and the gene in the middle:
Order of genes:
vg - a - pr
Gene in the middle:
a
How to find gene order?
To determine the order of the genes, use the following information:
The distance between vg and pr is 12.5 m.u.
The distance between a and pr is 44.7 m.u.
The distance between a and vg is 32.2 m.u.
From this information, the distance between vg and a is 32.2 m.u., which is the sum of the distances between vg and pr and a and pr. This means that the genes must be in the order vg - a - pr.
The gene in the middle is a because it is between vg and pr.
Therefore, the order of the genes on chromosome II is vg - a - pr, and the gene pr" (purple eyes) in the middle.