asked 204k views
0 votes
which step of dna isolation requires a nacl salt solution to stabilize dna molecules and prevents degredation?

2 Answers

5 votes

Final answer:

The step of DNA isolation that requires a NaCl salt solution to stabilize DNA molecules and prevent degradation is the step of salt extraction.

Step-by-step explanation:

The step of DNA isolation that requires a NaCl salt solution to stabilize DNA molecules and prevent degradation is the step of salt extraction. Salt extraction dissociates most of the proteins from the chromatin, allowing for the isolation of DNA. When a low salt extract is centrifuged and the pellet is resuspended, the remaining chromatin appears as beads on a string, with the DNA-wrapped nucleosomes as the beads. The NaCl salt solution stabilizes the DNA molecules and prevents their degradation during the isolation process.

answered
User Pretty Angela
by
7.8k points
4 votes

Answer: -Precipitation is the step of DNA isolation that requires a NaCl salt solution to stabilize DNA molecules and prevents degradation.

Explanation:

more info: it separates the freed DNA from the cellular debris. It involves the use of sodium (Na+) ions to neutralize any negative charge in DNA molecules, making them less water-soluble and more stable.

Welcome to Qamnty — a place to ask, share, and grow together. Join our community and get real answers from real people.